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ywpJ [2019-02-22 11:43:46]
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ywpJ [2019-02-22 11:43:46]

putative [SW|HAD superfamily] phosphatase
locus
BSU36290
pI
4.88
mw
31.80 kDa
protein length
285 aa Sequence Blast
gene length
858 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    3,738,343 → 3,739,200

    The protein

    Protein family

  • [SW|HAD superfamily]
  • Cof family (according to Swiss-Prot)
  • Modification

  • phosphorylated on Arg-258 [Pubmed|22517742]
  • Structure

  • [PDB|1NRW]
  • Expression and Regulation

    Operons

    genes
    [gene|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|glcR]-[gene|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC]
    description
    [Pubmed|30782637,22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|30782637], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|GlcR]: repression, [pubmed|30782637], in [regulon|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|GlcR regulon]
  • regulation

  • induced by phosphosugar stress [Pubmed|30782637]
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    Biological materials

    Mutant

  • MGNA-A531 (ywpJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/531 NBRP B. subtilis, Japan]
  • BKE36290 (Δ[gene|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCGCAATTAATTTCACGT, downstream forward: _UP4_TAAGTATATGTGCTGCCACA
  • BKK36290 (Δ[gene|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCGCAATTAATTTCACGT, downstream forward: _UP4_TAAGTATATGTGCTGCCACA
  • References

  • 22383849,22517742